Journal: Oncology Letters

Article Title: An m6A-programmed cell death signature predicts prognosis and identifies STK25 as a therapeutic target in colon adenocarcinoma

doi: 10.3892/ol.2026.15610

Figure Lengend Snippet: METTL3/YTHDC1 regulates STK25 mRNA stability via m6A modification. (A) Bar plot showing Spearman correlation coefficients between STK25 and 23 m6A regulators. Red bars indicate positive correlations; blue bars indicate negative correlations and color intensity reflects correlation strength. (B) Credibility scores of m6A regulators predicted to target STK25, as obtained from the RM2Target database. (C) Reverse transcription-qPCR analysis of STK25 mRNA levels in RKO and LOVO cells following knockdown of METTL3 or YTHDC1. (D) RIP assay using METTL3 and YTHDC1 antibodies, showing enrichment of STK25 mRNA compared with IgG control. (E) MeRIP-qPCR analysis of m6A modification on STK25 mRNA in control and METTL3-knockdown cells, demonstrating decreased m6A enrichment upon METTL3 knockdown. * P<0.05, ** P<0.01, ***P<0.001, ****P<0.0001. qPCR, quantitative PCR; RIP, RNA immunoprecipitation; MeRIP, methylated RNA immunoprecipitation; METTL3, methyltransferase-like 3; YTHDC1, YTH domain containing 1; STK25, serine/threonine kinase 25; si, small interfering RNA; NC, negative control.

Article Snippet: Briefly, magnetic beads were incubated overnight at 4°C with 5 μg of antibodies against METTL3 (Cohesion Biosciences Limited; cat. no. CQA1783) or YTHDC1 (Abcam; cat. no. ab264375) or normal IgG as a negative control (Beyotime Biotechnology, cat. no. A7016).

Techniques: Modification, Reverse Transcription, Knockdown, Control, Real-time Polymerase Chain Reaction, RNA Immunoprecipitation, Methylation, Small Interfering RNA, Negative Control

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: Analysis of m 6 A methylase expression levels in chicken tissue. (A) The IMF content in the breast muscle at 42 (42 D), 126 (126 D), and 180 (180 D) days of age ( n = 5). (B) m 6 A levels in breast muscle across three developmental stages ( n = 5). (C) The expression levels of methyltransferase and demethylase in the breast muscle across three developmental stages ( n = 5). (D) Tissue expression profiles of METTL3 in three developmental stages ( n = 3). Different capital letters indicate extremely significant differences between groups ( P < 0.01).

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Expressing

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: Overexpression of METTL3 inhibits IMPA cell proliferation. (A) RT-qPCR and (B) western blot detection of METTL3 overexpression efficiency in IMPA cells ( n = 3). (C) Overexpression of METTL3 inhibits mRNA expression of proliferation-related genes ( n = 3). (D) CCK-8 and (E) EdU assays were used to detect the proliferation activity of IMPA cells after METTL3 overexpression (scale bar: 100 μm).

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay, Activity Assay

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: Interference with METTL3 promotes IMPA cell proliferation. (A) Interference efficiency of METTL3 in IMPA cells ( n = 3). (B) Interference with METTL3 promotes the expression of proliferation-related genes ( n = 3). (C) CCK-8 and (D) EdU assays to detect the proliferation activity of IMPA cells after METTL3 interference (scale bar: 100 μm).

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Expressing, CCK-8 Assay, Activity Assay

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: METTL3 inhibits adipogenesis in IMPA cells. (A) mRNA expression of adipogenesis-related genes after overexpression and interference of METTL3 ( n = 3). (B) Oil red O and (C) BODIPY staining results of IMPA cells after overexpression and interference of METTL3 (scale bar: 100 μm). (D) Quantitative results of Oil Red O and BODIPY staining.

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Expressing, Over Expression, Staining

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: Screening of m 6 A modification genes regulated by METTL3 . mRNA expression of m 6 A-modified genes after overexpression of METTL3 in (A) IMPA and (B) APA cells ( n = 3). (C) mRNA expression of PCYT1A in the breast muscle at three developmental stages ( n = 5). (D) m 6 A-qPCR detection of m 6 A modification levels of PCYT1A -3′ UTR in the breast muscle at three developmental stages ( n = 5).

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Modification, Expressing, Over Expression

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: Expression of METTL3 and PCYT1A during cell differentiation. (A) Changes in METTL3 expression in IMPA and APA cells at 0, 2, 4, 6, and 8 days of differentiation ( n = 3). Different capital letters indicate extremely significant differences between groups ( P < 0.01), and different lowercase letters indicate significant differences between groups ( P < 0.05). (B) Changes in PCYT1A expression in IMPA and APA cells at 0, 2, 4, 6, and 8 days of differentiation ( n = 3). (C) Correlation analysis between METTL3 and PCYT1A during IMPA and APA cell differentiation.

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Expressing, Cell Differentiation

Journal: Poultry Science

Article Title: The mechanism by which methylase METTL3 affects intramuscular fat deposition

doi: 10.1016/j.psj.2026.106670

Figure Lengend Snippet: METTL3 affects the mRNA stability and expression of PCYT1A by regulating its m 6 A modification. (A) Expression efficiency of METTL3-WT and METTL3-MUT in IMPA and APA cells ( n = 3). (B) RT-qPCR detection of the effect of METTL3-WT and METTL3-MUT on PCYT1A mRNA expression ( n = 3). (C) WB detection of METTL3 overexpression and interference effect on PCYT1A protein expression in IMPA cells ( n = 3). (D) RT-qPCR detection of the regulatory role of METTL3 interference on PCYT1A mRNA expression ( n = 3). (E) m 6 A-qPCR detection of the effect of METTL3 interference on PCYT1A m 6 A modification levels ( n = 3). (F) Half-life analysis of PCYT1A mRNA in IMPA and APA cells after METTL3 interference ( n = 3).

Article Snippet: Antibody information was shown as follows: METTL3 (Proteintech, 15073-1-AP, 1:1000), PCYT1A (Affinity, DF7927, 1:2000), and GAPDH (Abways, AB0037, 1:10000).

Techniques: Expressing, Modification, Quantitative RT-PCR, Over Expression

Journal: International Journal of Genomics

Article Title: m6A Methyltransferase METTL3 Regulates Inflammatory and Immune Microenvironments in Renal Cell Carcinoma via Modulation of the PI3K/AKT Pathway

doi: 10.1155/ijog/4975062

Figure Lengend Snippet: Detection of PD‐L1, HLA‐I, and CD8 + T‐cell expression to analyze the regulation of the 786‐O immune microenvironment by METTL3 through the PI3K/AKT pathway. ∗ indicates p < 0.05 compared to the blank group. Quantitative data are presented as mean ± SD from three independent experiments. Abbreviations: HLA‐I, human leukocyte antigen class I; PD‐L1, programmed death‐ligand 1.

Article Snippet: All gene constructs for abnormal METTL3 expression were designed and synthesized by Shanghai OBiO Technology Co., Ltd.

Techniques: Expressing